Award details

The anaerobic and aerobic C4-dicarboxylic transport system of Escherichia coli

Reference	D01841
Principal Investigator / Supervisor	Professor Simon C Andrews
Co-Investigators / Co-Supervisors	Professor J Guest, Professor Adrian Walmsley
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	141,294
Status	Completed
Type	Research Grant
Start date	06/06/1994
End date	06/06/1997
Duration	36 months

Abstract

Escherichia coli processes two homologous and independent C4- dicarboxylate membrane antiport proteins (GenA and GenF) which are distinct from the binding-protein dependent aerobic dicarboxylate transporter (the Dct system). The aim of the study is to investigate the structure and function of the anaerobic porters and to elucidate their relative roles. This will involve overproduction and purification of the proteins, kinetic analyses, topological investigations, and gene regulation studies. The genes encoding the aerobic system will also be cloned and sequenced enabling further analysis of the Dct system and comparison with the anaerobic transporters. If time permits, the possible existence of a third anaerobic dicarboxylate transporter will be investigated.

Summary

unavailable

Committee	Closed Committee - Agri-food (AF)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search