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Characterisation of transposon-tagged alleles of the tomato dwarf gene, and analysis and manipulation of dwarf gene function
Reference
D01834
Principal Investigator / Supervisor
Professor Jonathan Jones
Co-Investigators /
Co-Supervisors
Institution
University of East Anglia
Department
Sainsbury Laboratory
Funding type
Research
Value (£)
145,128
Status
Completed
Type
Research Grant
Start date
01/04/1994
End date
01/04/1997
Duration
36 months
Abstract
Weak alleles of the tomato dwarf gene have been used to breed compact tomato varieties. We have used targeted transposon mutagenesis with the maize Activator (Ac) element to isolate three independent mutations at the dwarf locus. There is good evidence that the gene is tagged in two of these mutations, because (i) germinal and somatic reversion is detected and (ii) DNA analysis suggests reversion is accompanied by Ac excision. The third mutation probably involves a deletion. We propose to (i) sequence the dwarf gene and transcript to obtain clues about its function (ii) characterize the basis of spontaneous tomato mutations in the gene (iii) carry out antisense experiments to engineer alterations in plant shape.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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