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Molecular characterisation of the role of galactose metabolism in the formation of the mycobacterial cell envelope
Reference
D01623
Principal Investigator / Supervisor
Professor Duncan Maskell
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Veterinary Medicine
Funding type
Research
Value (£)
149,742
Status
Completed
Type
Research Grant
Start date
11/07/1994
End date
06/04/1998
Duration
45 months
Abstract
Many of the virulence determinants of pathogenic mycobacteria have been attributed to their lipid-rich, highly complex, cell envelope. The cell envelope core consists of mycolic acids anchored via arabinogalactan chains to the peptidoglycan cell wall. We propose to clone and sequence the genes for mycobacterial galactose metabolism. Strategies used to clone the genes will include degenerate oligonucleotide polymerase chain reaction using mycobacterial DNA as template, and genetic complementation. Once cloned and sequenced the genes will be mutagenized with a view to preventing arabinogalactan synthesis. The effect of mutations in gal genes on cell surface structure and virulence of mycobacteria will then be investigated.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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