Award details

Clean technology through the engineered biosynthesis of polyketides

Reference	CT04273
Principal Investigator / Supervisor	Professor Sir David Hopwood
Co-Investigators / Co-Supervisors	Professor Kevin Booker-Milburn, Professor G Richard Stephenson
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	305,321
Status	Completed
Type	Research Grant
Start date	01/01/1995
End date	31/03/1999
Duration	51 months

Abstract

This project will use a combination of genetics, chemistry and biochemistry to identify the genes that control each step in the formation of the pyran rings of four benzoisochromonequinones and to explore the mechanisms of reactions catalysed by the gene products. Conventional gene replacement and a temperate bacteriophage-mediated gene disruption will be used to introduce targeted mutations in ORF's relevant to the biosynthetic pathways of Streptomyces coelicolor. After isolation and characterisation of polyketide bio- products, isotopically labelled intermediates will be synthesised for use in feeding experiments to confirm details of the biosynthetic pathways.

Summary

unavailable

Committee	Closed Committee - Plant & Microbial Sciences (PMS)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Clean Technology (EPSRC) (CT) [1993-1995]
Funding Scheme	X – not Funded via a specific Funding Scheme

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