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A step change in the application of PCR to the detection of bacteria
Reference
CEL04626
Principal Investigator / Supervisor
Dr G Stewart
Co-Investigators /
Co-Supervisors
Institution
University of Nottingham
Department
Sch of Biosciences
Funding type
Research
Value (£)
100,747
Status
Completed
Type
Research Grant
Start date
01/11/1995
End date
01/11/1997
Duration
24 months
Abstract
PCR can only interrogate a sample for a preselected target optimum. It would be preferable to determine if any contaminating bacteria were present in a sample, followed by identification. As part of a BBSRC FOOD grant (FG42/638) we have determined that `cold shock' genes are generically conserved in procaryotes. There exists a family in each bacterium and regions of high sequence conservation provide PCR primers for DNA amplification from any bacterium. Sequence specificity within the amplified DNA then provides for speciation. A patent on this technology has been filed and I am seeking to develop the scientific concepts further.
Summary
unavailable
Committee
Closed Committee - Agri-food (AF)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Cells RoPA (CELL) [1994]
Funding Scheme
X – not Funded via a specific Funding Scheme
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