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Programming the commitment of aerial hyphae to sporulation in Streptomyces coelicolor
Reference
CAD04380
Principal Investigator / Supervisor
Professor Keith Chater
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
John Innes Centre Department
Funding type
Research
Value (£)
127,777
Status
Completed
Type
Research Grant
Start date
14/08/1995
End date
14/08/1998
Duration
36 months
Abstract
1. By the sequencing, transcription analysis and targeted disruption of the (already cloned) whiA, H and J genes, we will complete the basic characterisation of a set of five known regulatory loci that together activate cytokinesis of aerial hyphae, leading to sporulation. 2. By SI mapping in defined null mutants transcriptional interdependence among the five genes will be evaluated. 3. By overexpression of E. coli appropriate whi gene products will be purified to raise antibodies (and for use in in vitro studies of DNA-protein (or protein-protein) interactions). 4. The effects of ectopic expression of the same whi genes in S. coelicolor will be evaluated, by Western blotting, S1 mapping and phenotypic analysis, to test in vivo the mechanistic hypotheses arising from 1-2.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Commitment and Determination (CAD) [1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
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