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Identification of cell surface molecules important for the formation and patterning of somites in the mouse
Reference
CAD04332
Principal Investigator / Supervisor
Professor Lesley Forrester
Co-Investigators /
Co-Supervisors
Institution
University of Edinburgh
Department
Inst of Stem Cell Research
Funding type
Research
Value (£)
121,600
Status
Completed
Type
Research Grant
Start date
08/01/1996
End date
31/03/1999
Duration
39 months
Abstract
The 'secretory trap', an approach based on the gene trap, will be used in mouse embryonic stem (ES) cells to create insertional mutations in genes that encode secreted and membrane- spanning proteins. In preliminary work, we identified three genes expressed in the somite lineage; a novel laminin related to unc-6 in C. elegans, the receptor tyrosine kinase sek, and the receptor-linked protein tyrosine phosphatase PTPk. The effect of these insertional mutations on the formation and subsequent patterning of somites will be investigated. The major aim of this project will be develop a lineage-specific screen based on in vitro differentiation of ES cells into somitic mesoderm. In this way, large numbers of secretory trap insertions may be screened to find those relevant to the process of somitigenesis.
Summary
unavailable
Committee
Closed Committee - Genes & Developmental Biology (GDB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
Commitment and Determination (CAD) [1995]
Funding Scheme
X – not Funded via a specific Funding Scheme
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