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The protein-protein and protein-nucleotide interactions that establish cell commitment in sporulating Bacillus subtilis

Reference	CAD04313
Principal Investigator / Supervisor	Dr M Yudkin
Co-Investigators / Co-Supervisors	Professor Jeffery Errington
Institution	University of Oxford
Department	Biochemistry
Funding type	Research
Value (£)	158,544
Status	Completed
Type	Research Grant
Start date	01/07/1995
End date	01/11/1998
Duration	40 months

Abstract

The activity of sigma F, which is responsible for cell commitment during sporulation, is regulated by interactions between sigma F, SpoIIAB (which inhibits its activity) and SpoIIAA (which counteracts SpoIIAB). We plan to use physical and enzymological techniques to study 1) the binding of ADP and ATP to the proteins; 2) the phosphorylation of SpoIIAA; 3) changes in protein conformation in response to nucleotide binding or phosphorylation; and 4) the formation of complexes between the proteins, and the effect of nucleotides on these complexes. We also plan to use immunoprecipitation to determine in sporulating cells the presence of the protein complexes, the ratio of SpoIIAA to SpoIIAA- phosphate, and the ratio of SpoIIAA, to SpoIIAB.

Summary

unavailable

Committee	Closed Committee - Genes & Developmental Biology (GDB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	Commitment and Determination (CAD) [1995]
Funding Scheme	X – not Funded via a specific Funding Scheme

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