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High resolution imaging of viral entry into living cells using scanning surface confocal microscopy

Reference	C20199
Principal Investigator / Supervisor	Professor Yuri Korchev
Co-Investigators / Co-Supervisors	Professor David Klenerman
Institution	Imperial College London
Department	Dept of Medicine
Funding type	Research
Value (£)	406,018
Status	Completed
Type	Research Grant
Start date	01/04/2004
End date	31/03/2007
Duration	36 months

Abstract

We have developed a novel method, scanning surface confocal microscopy, to simultaneously obtain high resolution topographic and fluorescence images of the surface of cells (PNAS 2002, 99: 16022). We have demonstrated this method can be used to follow viral entry into live cells. We seek to build on this work to describe initial binding and entry of virus-like particles and adeno- and adeno-associated viruses by making dynamic measurements on live cells. We will relate the viral entry point to cytoskeletal structures and endocytic vesicles by performing two colour fluorescence experiments to label both the virus and the structure. We will also identify surface features that influence viral entry and aim to relate the route of entry to infectivity. This work should provide new details of the fundamentals of virus entry and help the optimisation of gene therapy strategies.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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