Award details

Probing nascent peptide: ribosome exit tunnel interactions

Reference	C20035
Principal Investigator / Supervisor	Professor Martin Ryan
Co-Investigators / Co-Supervisors	Dr Jeremy Brown, Dr Pablo De Felipe
Institution	University of St Andrews
Department	Biology
Funding type	Research
Value (£)	204,553
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/09/2006
Duration	36 months

Abstract

The FMDV 2A peptide catalyses co-translational cleavage at its own C-terminus in eukaryotic cells. This project aims to understand the mechanism of this intra-ribosomal polypeptide scission reaction. Using the observation that 2A is active in the genetically tractable S. cerevisiae we will undertake screens to establish both critical features of 2A that contribute to, and host cell factors that are required for, cleavage. Further, based on similarity between 2A and bacterial peptides that cause translation we will undertake experiments to identify 2A variants that function in the context of prokaryotic cells. Finally we will establish conditions for trapping and purifying ribosomes containing 2A thereby opening the door to structural studies. (Joint with grant number 20418).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C20418 Probing nascent peptide: ribosome exit tunnel interactions

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