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Signal output by the adaptor protein Grb2: regulation by Shc A and phosphorylation
Reference
C19979
Principal Investigator / Supervisor
Professor Stephen Dilworth
Co-Investigators /
Co-Supervisors
Institution
Imperial College London
Department
Dept of Medicine
Funding type
Research
Value (£)
179,326
Status
Completed
Type
Research Grant
Start date
01/04/2004
End date
31/03/2007
Duration
36 months
Abstract
Creation of a ShcA-Grb2 interaction is a key component of mitogenic signalling, but the mechanisms involved in regulating signal output from the complex are unclear. We have shown that two phosphotyrosine sites in ShcA interact with Grb2 and each has distinctive in vivo functions. Here a biosensor assay will be used to establish the hypothesis that association between ShcA and Grb2 influences binding to downstream signalling molecules, and ascertain how feedback phosphorylation regulates these interactions. We have also reported that Gab1 is bound to MT associated Grb2, so to prove the premise that this plays a part in signalling, Gab1 expression will be disrupted by RNA interference, and the effects on MT induced mitogenesis determined.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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