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The functional significance of CD40/CD40L interactions between Leukocytes and primary human liver cells: studies using a novel co culture system

Reference	C19953
Principal Investigator / Supervisor	Dr Simon Afford
Co-Investigators / Co-Supervisors	Professor David Adams, Professor Patricia Lalor
Institution	University of Birmingham
Department	Medical Sciences - Medicine
Funding type	Research
Value (£)	227,568
Status	Completed
Type	Research Grant
Start date	26/01/2004
End date	25/02/2007
Duration	37 months

Abstract

CD40 has a cell-specific role in determining the livers response to injury. Whereas activation of CD40 on hepatocytes leads to apoptosis, its activation on sinusoidal endothelium stimulates proliferation and chemokine and adhesion molecule expression. A model of the hepatic sinusoid in which human sinusoidal endothelium is co-cultured with liver-derived fibroblasts, hepatocytes and leukocytes will be used to investigate how CD40-mediated endothelial activation regulates leukocyte recruitment from blood flow. The ability of soluble versus cell bound CD40 ligand, to activate CD40 will be compared. The transcription factors and genes activated in response to engagement of endothelial CD40 by different modes of ligand presentation will be determined using cell transfections, transcription factor assay, cDNA microarray and proteomic analysis.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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