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Characterisation of the EB1 family of microtubule-associated proteins in plants

ReferenceC19933
Principal Investigator / Supervisor Professor Clive Lloyd
Co-Investigators /
Co-Supervisors
Professor John Doonan
Institution John Innes Centre
DepartmentJohn Innes Centre Department
Funding typeResearch
Value (£) 213,258
StatusCompleted
TypeResearch Grant
Start date 03/11/2003
End date 02/11/2006
Duration36 months

Abstract

Plant cells do not have microtubule organising centres. Without markers for MT nucleation we do not know how MT arrays are built, especially the cortical array essential for cell elongation. Promising pilot studies show that GFP-EB1 labels bodies at spindle poles (the classical location for MTOCs) and punctae on cortical MTs. Next we propose to clone the small multigene family. Labelling EB1 on plasma membrane disks with immunogold will allow us to visualise the MT nucleation sites and establish the orientation of cortical MTs. EB1 is known to form a complex and we will identify binding partners by affinity tags and proteomics. The developmental role of EB1 will be assayed by RNAi and by screening insertional mutant lines.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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