Award details

Genetic and biochemical analysis of a novel Tat protein export pathway in the archaeon Halobacterium salinarum

ReferenceC19762
Principal Investigator / Supervisor Dr Albert Bolhuis
Co-Investigators /
Co-Supervisors
Institution University of Warwick
DepartmentBiological Sciences
Funding typeResearch
Value (£) 194,772
StatusCompleted
TypeResearch Grant
Start date 05/01/2004
End date 04/01/2007
Duration36 months

Abstract

The Twin-arginine translocation (Tat) pathway, which has the unique ability to transport fully folded proteins, is involved in the transport of the majority of extracytoplasmic proteins in the halophilic archaeon Halobacterium salinarum. This is in stark contrast to most other organisms, which require the Tat pathway only for a small subset of proteins. Genome analysis clearly indicates that the halobacterial Tat-translocase is unique in terms of both composition and structure, and this project is aimed at: (i) genetic analysis of tat genes; (ii) purification of the translocase and determination of its subunit composition; and (iii) analysis of the mechanism of Tat-dependant transport through the development of in vitro translocation assays.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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