Award details

Nitrite reduction by Paracoccus pantotrophus cytochrome cd1: structural mobility and mechanism of a NO generating enzyme

Reference	C19434
Principal Investigator / Supervisor	Dr Nicholas James Watmough
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Biological Sciences
Funding type	Research
Value (£)	180,084
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/09/2006
Duration	36 months

Abstract

The aim of this programme is to elucidate the detailed mechanism of the physiological nitrite reductase reaction of this enzyme. In particular we wish to understand how the release of the product, nitric oxide, is controlled by the specialised d1 heme active site, rather than being trapped there as a stable ferrous heme-nitric oxide adduct. The proposed work will draw upon new knowledge of conformations that can be adopted by cytochrome cd1 as well as considerably extend our analysis of the nitrite reductase reaction. In order to do this we need to deploy a wide range of methods, including mutagenesis, x-ray crystallography and a number of different, but complementary time-resolved spectroscopies. (Joint with grant number 19430).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C19430 Nitrite reduction by paracoccus pantotrophus cytochrome cd1; structural mobility and mechanism of a NO generating enzyme

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