Award details

Enzymatic activity of a mono-haem c-type cytochrome. Cytochrome c' is a nitric oxide reductase

ReferenceC19243
Principal Investigator / Supervisor Professor James Moir
Co-Investigators /
Co-Supervisors
Institution University of York
DepartmentBiology
Funding typeResearch
Value (£) 181,472
StatusCompleted
TypeResearch Grant
Start date 20/10/2003
End date 19/03/2007
Duration41 months

Abstract

In previous BBSRC-funded work we demonstrated that the bacterial mono-haem cytochrome c from Rhodobacter capsulatus possesses a nitric oxide reductase activity. NO detoxification is required in diverse biological environments, and cytochrome c is widely distributed among the proteo-bacteria. In this project we will express and purify cytochrome c and its putative electron donor protein CybP from R. capsulatus in order to develop a biochemical assay for NO reduction by this system, characterise the enzymological properties of cytochrome c and measure the products of NO reduction. Also, we will investigate the enzymatic properties of the cytochrome c from pathogenic bacterium Neisseria meningitidis.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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