Award details

Trafficking and targeting of peroxisomal membrane protein Pex2p

Reference	C19030
Principal Investigator / Supervisor	Professor Alison Baker
Co-Investigators / Co-Supervisors	Professor Christopher Hawes, Dr Imogen Sparkes
Institution	University of Leeds
Department	Plant Biochemistry & Biotechnology
Funding type	Research
Value (£)	35,512
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	31/03/2006
Duration	36 months

Abstract

This proposal aims to establish whether a biogenetic connection exists between the endoplasmic reticulum and peroxisomes in higher plants. Using the peroxisomal membrane protein Pex2p tagged with fluorescent proteins we will test whether trafficking is via the ER by using a combination glycosylation assays and pulse chasing with photoactivatable fluorescent proteins. Expression of mutant regulatory proteins in combination with a photobleaching strategy will test whether ER exit is COPII dependent. If not, a screen will be undertaken to search for mutants that mislocalise Pex2p to the ER or cytoplasm to identify components of this novel pathway. If trafficking proceeds by know pathways we will dissect the targeting signal of Pex2p instead. (Joint with grant 19029).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C19029 Trafficking and trargeting of the peroxisomal membrane protein Pex2p

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