Award details

Study of ion channels in sub-cellular structures using automated high resolution scanning patch clamp

Reference	C19021
Principal Investigator / Supervisor	Professor Yuri Korchev
Co-Investigators / Co-Supervisors	Professor David Klenerman
Institution	Imperial College London
Department	Dept of Medicine
Funding type	Research
Value (£)	281,688
Status	Completed
Type	Research Grant
Start date	13/10/2003
End date	12/10/2006
Duration	36 months

Abstract

We have recently developed a high-resolution patch-clamp technique for mapping ion channels and shown the feasibility of patching sub-cellular structures which were not possible to study with any conventional methods. We now seek funds to follow on this work and build an automated high-resolution instrument. We will then use this to study and characterise the ion channels in sperm involved in the acrosome reaction, the voltage gated Ca2+ and Ca2+ sensitive K+ channels in dendritic synapses which are involved in neuronal signalling and Na+, K+ and C1 channels in the microvillus region of kidney epithelial cells that are responsible for sodium reabsorption and volume regulation. Each of these studies has specific technical issues that need to be addressed so that the final methodology should be widely applicable in biomedical research enabling rapid and reliable patching of sub- cellular features on cell cultures or tissue slices.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search