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The mechanism of the ER retention of 5-HT3 receptors and the site of receptor assembly in primary neurons in culture
Reference
C18896
Principal Investigator / Supervisor
Dr Chris Connolly
Co-Investigators /
Co-Supervisors
Institution
University of Dundee
Department
College of Life Sciences
Funding type
Research
Value (£)
233,828
Status
Completed
Type
Research Grant
Start date
24/03/2003
End date
23/08/2006
Duration
41 months
Abstract
We have shown recently that recombinantly expressed 5-HT3A receptors are functionally expressed at the cell surface. In contrast, 5-HT3B receptors are retained in the ER until rescued by the 3A subunit to form heteromeric surface receptors. We have now identified an ER-retention signal within the 5-HT3B subunit. When transferred to 5-HT3A, the subunit is ER-retained until rescued by the wild-type 3A subunit. However, other ER-retention signals exist within the 3B. The use of fluorescently tagged, ER- retained, 5HT3 receptor constructs will permit us to study the distribution of unassembled subunits and the site of receptor assembly to determine whether receptors are recruited to synapses from the cell body or are synthesised locally.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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