Award details

The signalling and mechanism of apoptotic cell extrusion from simple epithelia

Reference	C18722
Principal Investigator / Supervisor	Dr Louise Cramer
Co-Investigators / Co-Supervisors	Professor Jody Rosenblatt
Institution	University College London
Department	MRC Laboratory of Molecular Cell Biology
Funding type	Research
Value (£)	157,848
Status	Completed
Type	Research Grant
Start date	01/07/2003
End date	30/06/2006
Duration	36 months

Abstract

We have discovered a novel mechanism for how a dying cell is removed from a simple epithelium while still maintaining its barrier function. An epithelial cell destined for apoptosis signals its live epithelial neighbours to form an actin and myosin purse- string around the dying cell that contracts to extrude the dying cell out of the layer. Using cultured epithelial monolayers we plan to dissect the signalling and mechanism of apoptotic cell extrusion. We will use an RNAi screen and 2-D gel-based proteomics to identify the signal for extrusion. To analyse how the actin ring forms and contracts we will image extrusion using GFP-tagged cytoskeletal proteins and we will use RNAi and inhibitory drugs to identify other proteins required for extrusion.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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