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Functional studies of the Arabidopsis tic complex
Reference
C18638
Principal Investigator / Supervisor
Professor Paul Jarvis
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Biology
Funding type
Research
Value (£)
200,636
Status
Completed
Type
Research Grant
Start date
14/04/2003
End date
14/04/2006
Duration
36 months
Abstract
Arabidopsis Tic110, Tic40 and ClpC-V knockout (ko) mutants have chloroplast biogenesis defects. These mutants will be characterised to elucidate the roles played by the proteins in chloroplast protein import, particularly in relation to import motor function. Basic characterisation of the proteins (expression and localisation) and mutants (complementation; in vitro import studies; chloroplast proteomics) will be completed in the first 12 months. Interactions between the three proteins will be studied genetically (double mutants; trans-complementation) and at the molecular level (yeast two-hybrid; co-immunoprecipitation). Detailed mutant studies will assess the possible role of each protein in driving import. Finally, to identify novel loci involved in chloroplast protein import, a screen for Tic40ko second-site suppressors will be conducted.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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