Award details

Local modification of the human mucosal antibody response

Reference	C18415
Principal Investigator / Supervisor	Professor Jo Spencer
Co-Investigators / Co-Supervisors
Institution	King's College London
Department	Immunobiology
Funding type	Research
Value (£)	188,644
Status	Completed
Type	Research Grant
Start date	01/10/2003
End date	30/09/2006
Duration	36 months

Abstract

This proposal is formulated around our published and preliminary studies of human tissues and high profile studies using murine models. We wish to test the hypothesis that the human mucosal antibody response is modified by class switch recombination (CSR) and somatic hypermutation (SH) in the mucosal microenvironment outside organised lymphoid tissue, in which case it may be unselected and T-cell independent. This knowledge is essential for rational design of mucosal vaccines. Two approaches are proposed. Firstly microdissection of tissue sections, PCR and sequence analysis to look for prerequisites and products of CSR and SH in the mucosa in situ. Secondly to identify the factors required for this CSH and SH by in vitro culture of purified human lamina propria derived B cells with defined accessory cells and cytokines known to regulate CSR and SH in other systems.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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