Award details

The role a novel phosphatase in skeletal mineralisation and bone development

Reference	C18180
Principal Investigator / Supervisor	Professor Colin Farquharson
Co-Investigators / Co-Supervisors	Dr James McWhir
Institution	The Roslin Institute
Department	Division of Integrative Biology
Funding type	Research
Value (£)	159,876
Status	Completed
Type	Research Grant
Start date	17/02/2003
End date	17/02/2006
Duration	36 months

Abstract

PHOSPHO1 is a novel phosphatase that is highly expressed in mineralising tissues and cells. This project aims to obtain evidence to support our hypothesis that PHOSPHO1 is involved in generating inorganic phosphate for skeletal mineralisation. The function of PHOSPHO1 will first be addressed using an antisense approach to inhibit its expression in human osteoblast-like cells and in a murine chondrogenic cell line, and determining the effect that this has on matrix mineralisation. We will also establish the phenotypic consequences of PHOSPHO1 null-allele murine embryonic stem cells. These approaches will enable us to identify the developmental stages at which PHOSPHO1 is activated and its role in mineralisation. The null-allele ES cells will also be used to generate null-allele mice to determine the effects of knocking-out PHOSPHO1 on the skeletal biology of the whole animal.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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