Award details

Biochemistry of plant AcylCoA binding proteins using a targeted proteomics approach

Reference	C18065
Principal Investigator / Supervisor	Dr Rob Field
Co-Investigators / Co-Supervisors
Institution	University of East Anglia
Department	Chemistry
Funding type	Research
Value (£)	189,684
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	30/09/2005
Duration	30 months

Abstract

Acyl CoA dependent enzymes serve essential roles in cellular metabolism, however, many of the enzymes remain to be identified and isolated. We aim to combine expertise in chemistry and enzymology to build a generic technology, using photoaffinity labelling and proteomic analysis, aimed at identification of acyl CoA dependent enzymes and their substrate binding sites. We will use this to: (1) identify the acyl binding site of soluble glycerol-3-phospate acyltransferase (G3PAT), (2) identify and clone the plant membrane bound G3PAT and the anthocyanin 6-aromatic acyltransferaese, and (3) isolate binding site peptides of acyl CoA-utilising enzymes using new affinity tags and investigate their application to complex mixtures of proteins. (Joint with grant 17862)

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C17862 Biochemistry of Acyl CoA dependent proteins in plants: a targeted proteomics approach

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