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Abscisic acid and nitric oxide signalling in Arabidopsis thaliana guard cells: cyclic cGMP synthesis and protein kinses activation

Reference	C17932
Principal Investigator / Supervisor	Dr John Travers Hancock
Co-Investigators / Co-Supervisors	Dr Peter Morris, Professor Steven Neill
Institution	University of the West of England
Department	Faculty of Health and Applied Sciences
Funding type	Research
Value (£)	219,284
Status	Completed
Type	Research Grant
Start date	01/05/2003
End date	31/08/2006
Duration	40 months

Abstract

We have shown that guard cells generate nitric oxide (NO) in response to abscisic acid (ABA), and that this NO is required for stomatal closure. We have good evidence that ABA and NO-mediated stomatal closure require the synthesis and action of cyclic GMP (cGMP). In addition, ABA and NO activate a protein kinase (PK) with the characteristics of a MAP kinase. This project will characterise the role of cGMP and the NO-activated PK in ABA-and NO-mediated stomatal closure in Arabidopsis thaliana, using a combination of physiological, biochemical, genetic and molecular approaches. We will determine ABA, NO and cGMP responses in wild type plants and various mutants. We will identify and quantify cGMP in Arabidopsis guard cell protoplasts (GCPs) using mass spectrometry and radioimmunoassay. We will identify the NO-activated PK in Arabidopsis GCPs and generate antisense plants in which the expression of this PK is reduced specifically in guard cells.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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