Award details

Identification maintenance and expression of plastid DNA minicircles in dinoflagellate algae

Reference	C17881
Principal Investigator / Supervisor	Professor Christopher Howe
Co-Investigators / Co-Supervisors
Institution	University of Cambridge
Department	Biochemistry
Funding type	Research
Value (£)	197,836
Status	Completed
Type	Research Grant
Start date	01/10/2002
End date	01/10/2005
Duration	36 months

Abstract

Dinoflagellate algae have recently been shown to have an anomalous chloroplast genome fragmented into minicircles, each with a single gene (and sometimes two and sometimes none). We will extend our characterisation of the coding complement of the chloroplast of the dinoflagellate Amphidinium operculatum, using PCR, transposomics and sequencing to see if the genome is limited to encoding proteins of the light reactions of photosynthesis and understand how the aberrant non-coding minicircles arise. This will be complemented by a proteomic analysis, which will also indicate whether editing occurs. We will develop a dinoflagellate plastid transformation system and use it to identify regions important in minicircle replication and gene expression.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search