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Evaluating the motor enzyme activity of the type III restriction endonucleases using a novel scanning probe microscope

Reference	C17872
Principal Investigator / Supervisor	Professor Mervyn Miles
Co-Investigators / Co-Supervisors	Dr Massimo Antognozzi, Professor Mark Dominik Szczelkun
Institution	University of Bristol
Department	Physics
Funding type	Research
Value (£)	173,128
Status	Completed
Type	Research Grant
Start date	01/09/2003
End date	31/08/2005
Duration	24 months

Abstract

The type III restriction endonucleases cleave DNA by communicating between the two distant recognition sites dependent upon ATP hydrolysis. To unravel the mechanism of these motor enzymes, we will use a transverse dynamic force microscope to examine protein- nucleic acid interactions at the single molecule level. This will be achieved in two ways: (1) by imaging surface-attached nucleoprotein assemblies at different stages of the reaction; and (2) by specifically attaching DNA between the surface and the probe to allow the viscoelastic response to be determined during the reaction. This proposal will not only increase our knowledge of DNA communications but also exploits a cross-discipline collaboration to develop an emerging methodology that will benefit biological research as a whole.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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