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The multiple roles of the magnesium chelatase H subunit in signalling regulation and biosynthesis

Reference	C17866
Principal Investigator / Supervisor	Krebs Professor of B Christopher Hunter
Co-Investigators / Co-Supervisors	Dr P Davison, Professor Julie Gray
Institution	University of Sheffield
Department	Molecular Biology and Biotechnology
Funding type	Research
Value (£)	201,500
Status	Completed
Type	Research Grant
Start date	01/10/2002
End date	01/10/2005
Duration	36 months

Abstract

Magnesium chelatase lies at the branchpoint in tetrapyrrole biosynthesis, and catalyses the insertion of magnesium into protoporphyrin. The H subunit of magnesium chelatase is proposed to have a role in regulating flux down the chlorophyll pathway, and in signalling between the plastid and the nucleus to co-ordinate gene expression. We propose to quantify levels of transcript, protein, and Mg chelatase activity and to establish the location of the Mg chelatase subunits within the chloroplast. We will examine the effects of the gun mutations on the function of the Mg chelatase H subunit and search for H-Protoporphyrin and H-MgProtoporphyrin complexes in Arabidopsis. Diurnally cycled, antisense and gun mutant Arabidopsis plants will be used as sources of experimental material. The overall aim is to construct a coherent model of the multiple roles of H in biosynthesis and regulation.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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