Award details

The specificity and basis of GPCR hetero - oligomerisation

Reference	C17856
Principal Investigator / Supervisor	Professor Graeme Milligan
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	177,000
Status	Completed
Type	Research Grant
Start date	15/02/2003
End date	15/02/2006
Duration	36 months

Abstract

Recent evidence suggests that G protein-coupled receptors are internalised into cells in response to agonist ligands as dimers or oligomeric complexes. Using multiplexed combinations of co- expressed receptors tagged with a novel, pH sensitive, cynanine dye and various fluorescent proteins we will explore this concept and the specificity of GPCR homo and hetero-dimerisation. By constructing forms of a receptor that can only be activated by a synthetic ligand that does not bind to the wild type receptor we will also explore if both partners of a receptor homo-dimer must bind ligand to induce internalisation. Mutations of receptors that alter the pathways of agonist-induced internalisation will also be used to assess if such mutants can form dimers with wild type receptors and, if so, which pathways are dominant in determining the phenotype of internalisation. These studies will develop novel techniques to explore the basis and specificity of receptor oligomerisation.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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