BBSRC Portfolio Analyser
Award details
Myosin kinetics and processivity
Reference
C17852
Principal Investigator / Supervisor
Professor Clive Bagshaw
Co-Investigators /
Co-Supervisors
Institution
University of Leicester
Department
Biochemistry
Funding type
Research
Value (£)
214,596
Status
Completed
Type
Research Grant
Start date
01/03/2003
End date
01/03/2006
Duration
36 months
Abstract
Understanding the mechanism of chemical-mechanical energy transduction in actomyosin systems requires both a knowledge of the structural features of the proteins as well as defining their kinetics of interaction. We will investigate the kinetics of myosins from the II, V and VI classes with actin filaments using sensitive fluorescence microscope assays to elucidate the coupling of ATPase activity with the crossbridge cycle and to define the characteristics that lead to processive motion. Fluorescence polarisation methods will be used to define the path of motion along the actin filament. These studies will be extended to mutant myosins that are disfunctional in vivo in order to correlate in vitro properties with their biological roles.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
I accept the
terms and conditions of use
(opens in new window)
export PDF file
back to list
new search