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Post-translational modifications and the function of regulators of G protein signalling

Reference	C17789
Principal Investigator / Supervisor	Professor Graeme Milligan
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	204,540
Status	Completed
Type	Research Grant
Start date	01/04/2003
End date	01/04/2006
Duration	36 months

Abstract

Regulators of G protein Signalling (RGS) proteins play key roles in controlling the kinetics of deactivation of signalling events transduced by heterotrimeric G proteins. Although the RGS proteins are known to be targets for a variety of post-translational modifications the roles of such alterations are poorly understood. This reflects that to date many key studies have only been performed with bacterially expressed, unmodified recombinant RGS proteins. By directly linking together G protein - coupled receptor and a range of wildtype and mutated RGS proteins these studies will explore the function of such modifications under conditions in which expression levels of the RGS proteins can be monitored and effective targeting to the plasma membrane ensured. Detailed enzyme kinetic analysis of the activation and deactivation of co-expressed G proteins will provide quantitative analysis of the basis and selectivity of RGS-G protein interactions.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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