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Structure-function analyses of the phototropin blue light receptors

Reference	C17551
Principal Investigator / Supervisor	Professor John Christie
Co-Investigators / Co-Supervisors
Institution	University of Glasgow
Department	Institute of Biomedical & Life Sciences
Funding type	Research
Value (£)	185,228
Status	Completed
Type	Research Grant
Start date	01/12/2002
End date	01/12/2005
Duration	36 months

Abstract

UV-A:blue light is an important environmental factor controlling plant growth and development. The phototropins (phot1 and phot2) are blue light receptor kinases that regulate both phototropism and light-induced chloroplast movement in Arabidopsis. The aim of this project is to further characterise the photochemical and biochemical properties of these photo receptors and to understand how their activities are regulated by light. We will: (i) Use site- directed, and random mutagenesis to identify regions of the chromophore-binding (LOV) domains important for light sensing and or light activation; (ii) Examine the activities of phot1 and phot2 expressed in insect cells and determine whether they exhibit different properties. (iii) Perform domain replacement studies to further investigate the individual roles of LOV1 and LOV2 in regulating phototropin function.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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