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Sorting of model proteins to plant vacuoles: molecular characterisation of receptors and transport intermediates

Reference	C17404
Principal Investigator / Supervisor	Professor Lorenzo Frigerio
Co-Investigators / Co-Supervisors	Professor Lynne Roberts
Institution	University of Warwick
Department	Biological Sciences
Funding type	Research
Value (£)	184,636
Status	Completed
Type	Research Grant
Start date	01/08/2002
End date	01/08/2005
Duration	36 months

Abstract

We propose to study the pathways of vacuolar transport of two model storage proteins: ricin and Ricinus communis 2S albumin. These proteins reach the same matrix subcompartment of the protein storage vacuoles in Ricinus seeds by different, though yet poorly, characterised pathway(s) involving precursor accumulating (PAC) vesicles. From largely microscopical analysis, proalbumins are believed to enter PAC vesicles at the level of the endoplasmic reticulum, whereas proricin joins these vesicles after travelling through the Golgi. We propose to dissect these transport routes biochemically, both in Ricinus and in tobacco. We will isolate a sorting receptor for ricin and test the novel idea that clathrin-coated vesicles may be involved in transport from the Golgi to PAC. We will also perform proteomic analysis to elucidate the membrane protein complement of PAC vesicles.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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