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Sorting of model proteins to plant vacuoles: molecular characterisation of receptors and transport intermediates

Principal Investigator / Supervisor Professor Lorenzo Frigerio
Co-Investigators /
Professor Lynne Roberts
Institution University of Warwick
DepartmentBiological Sciences
Funding typeResearch
Value (£) 184,636
TypeResearch Grant
Start date 01/08/2002
End date 01/08/2005
Duration36 months


We propose to study the pathways of vacuolar transport of two model storage proteins: ricin and Ricinus communis 2S albumin. These proteins reach the same matrix subcompartment of the protein storage vacuoles in Ricinus seeds by different, though yet poorly, characterised pathway(s) involving precursor accumulating (PAC) vesicles. From largely microscopical analysis, proalbumins are believed to enter PAC vesicles at the level of the endoplasmic reticulum, whereas proricin joins these vesicles after travelling through the Golgi. We propose to dissect these transport routes biochemically, both in Ricinus and in tobacco. We will isolate a sorting receptor for ricin and test the novel idea that clathrin-coated vesicles may be involved in transport from the Golgi to PAC. We will also perform proteomic analysis to elucidate the membrane protein complement of PAC vesicles.


Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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