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Microtubule motor-dependent regulation of mitochondrial dynamics during apotosis

Reference	C16819
Principal Investigator / Supervisor	Professor Viki Allan
Co-Investigators / Co-Supervisors	Dr Jonathan Lane, Professor Philip Woodman
Institution	The University of Manchester
Department	Life Sciences
Funding type	Research
Value (£)	103,500
Status	Completed
Type	Research Grant
Start date	04/12/2001
End date	03/01/2004
Duration	25 months

Abstract

Mitochondria are centrally involved in co-ordinating events that regulate apoptosis. Current models suggest that Bax, a proapoptotic member of the Bcl-2 family of proteins, translocates to mitochondria after being locally activated. This is accompanied by changes in mitochondrial behaviour, including the clustering of mitochondria at the cell centre and the induction of a highly conserved apoptotic cascade. We will use time-lapse fluorescence and transmitted light microscopy to determine the sequence of events that occur during mitochondrial clustering, in terms of Bax recruitment, mitochondria activity, position and motility, and establish whether the movement is driven by cytoplasmic dynein. We will also investigate how wide-spread this clustering is during apoptosis triggered by a range of stimuli, and determine whether Bax translocation is required.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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