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A proteomics-based approach for understanding plant microtubule organization

Reference	C16743
Principal Investigator / Supervisor	Professor Clive Lloyd
Co-Investigators / Co-Supervisors	Professor John Doonan
Institution	John Innes Centre
Department	John Innes Centre Department
Funding type	Research
Value (£)	197,928
Status	Completed
Type	Research Grant
Start date	01/07/2002
End date	30/06/2005
Duration	36 months

Abstract

Microtubule-associated proteins (MAPs) help assemble MTs into 4 arrays vital to plant morphogenesis. Few are known; biochemical methods have been slow to characterise them and homology-based approaches have failed to detect some plant-specific MAPs. Proteomics offers a way ahead. In a pilot study we adapted to Arabidopsis our successful method for isolating carrot MAPs, demonstrating that representatives of several dozen proteins can be identified by mass spec peptide mapping. We propose to: (a) characterise these putative MAPs by epitope-tagging and determine cell location (b) isolate the complex linking MTs to the plasma membrane (c) test their role in MT organisation in vitro and in vivo.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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