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Analysis of cytoskeletal complexes in the cytokinetic apparatus the phragmoplast

Reference	C16706
Principal Investigator / Supervisor	Professor P Hussey
Co-Investigators / Co-Supervisors	Dr Hisako Igarashi, Professor Antony Slabas
Institution	Durham University
Department	Biological and Biomedical Sciences
Funding type	Research
Value (£)	200,888
Status	Completed
Type	Research Grant
Start date	13/01/2003
End date	13/01/2006
Duration	36 months

Abstract

The phragmoplast is a unique cytoskeletal structure responsible for cytokinesis in plants. We have developed a procedure for its isolation from synchronised tobacco cells. In addition, we have three different microtubule associated proteins: MAP 65, 190 and 215 all of which locate to this array. Using these tools we will affinity purify cytoskeletal complexes from the phragmoplast. These complexes will be fractionated on 2D gels and copurifying proteins sequenced using Q-TOF. We may identify known and novel proteins allowing us to obtain a clearer understanding of both the protein composition and the protein interactions within this framework. Identified sequences will be cloned and used to transform culture cells for comparative location studies. If time allows selected genes will be used to screen for insertion mutants.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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