Award details

Analysis of stress responses by expression profiling in novel human cell lines with impaired DNA repair pathways

Reference	C16678
Principal Investigator / Supervisor	Dr Christopher Jones
Co-Investigators / Co-Supervisors	Professor David Kipling
Institution	Cardiff University
Department	Division of Clinical Laboratory Science
Funding type	Research
Value (£)	312,040
Status	Completed
Type	Research Grant
Start date	14/10/2002
End date	13/04/2006
Duration	42 months

Abstract

Well-characterised DNA repair mechanisms guard our genomes but little is known of the effect of DNA damage in terms of the global response of the cell. This study will address these issues by measuring transcriptional changes in over 12,000 genes after DNA damage. We will also study the response in cells derived from individuals with genetic diseases that have repair defects and see whether lack of a correction pathway alters the response to DNA damage. We will create by gene targeting new cell lines with defects in other pathways that are not represented by human genetic disease due to embryonic lethality. Changes in a key protein p53 will be determined by looking at phosphorylation of the protein in these cell lines.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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