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Role of phosphorylation in the function of the circadian clock gene period 3

Reference	C16668
Principal Investigator / Supervisor	Professor Malcolm von Schantz
Co-Investigators / Co-Supervisors	Professor Simon Archer
Institution	University of Surrey
Department	Health and Medical Sciences
Funding type	Research
Value (£)	210,904
Status	Completed
Type	Research Grant
Start date	07/02/2002
End date	16/12/2005
Duration	46 months

Abstract

Mutations in the circadian clock gene period 3 (per3) alter period length in mice and cause sleep phase disorder in humans. The hPER3 sequence contains a series of tandem repeats absent in mPER3 that are putative recognition sites for casein kinase I (CK I) and glycogen synthase kinase 3 (GSK3). This region will be compared in a number of eutherians to investigate whether changes in this region relate to nocturnality and diurnality. We will then study the phosphorylation of PER3, by CK 1 and GSK3 and study the temporal expression of GSK3 in the mouse. Finally, we propose to express native, chimaeric and mutant PER3 protein in COS-7 cells and study differences in PER3 levels, localisation, and effect on the expression of the native per genes.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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