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A mechanistic study of the metabolisum of homocysteine by human endothelial cells; functional consequences for the vasculature

ReferenceC16643
Principal Investigator / Supervisor Professor Hilary Powers
Co-Investigators /
Co-Supervisors
Dr Gareth Evans
Institution University of Sheffield
DepartmentDivision of Clinical Sciences North
Funding typeResearch
Value (£) 104,072
StatusCompleted
TypeResearch Grant
Start date 01/05/2002
End date 01/05/2004
Duration24 months

Abstract

Homocysteine may pose an oxidant challenge in vivo and increase the risk of cardiovascular disease through this route. However, most in vitro studies have used unphysiological concentrations and forms of homocysteine. We will use a model system which will simulate conditions in vivo in that human endothelial cells in culture will export homocysteine with consequent autoxidation and the associated generation of reactive oxygen species, quantified through electron spin resonance spectroscopy. Oxidative modification of human low density lipoprotein in this system will be measured and the functional relevance of this determined through uptake studies with human monocyte-derived macrophages and the expression of the scavenger receptor. (Joint with grants 92/C16646 and 316/C16648).

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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