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A mechanistic study of the metabolisum of homocysteine by human endothelial cells; functional consequences for the vasculature

Reference	C16643
Principal Investigator / Supervisor	Professor Hilary Powers
Co-Investigators / Co-Supervisors	Dr Gareth Evans
Institution	University of Sheffield
Department	Division of Clinical Sciences North
Funding type	Research
Value (£)	104,072
Status	Completed
Type	Research Grant
Start date	01/05/2002
End date	01/05/2004
Duration	24 months

Abstract

Homocysteine may pose an oxidant challenge in vivo and increase the risk of cardiovascular disease through this route. However, most in vitro studies have used unphysiological concentrations and forms of homocysteine. We will use a model system which will simulate conditions in vivo in that human endothelial cells in culture will export homocysteine with consequent autoxidation and the associated generation of reactive oxygen species, quantified through electron spin resonance spectroscopy. Oxidative modification of human low density lipoprotein in this system will be measured and the functional relevance of this determined through uptake studies with human monocyte-derived macrophages and the expression of the scavenger receptor. (Joint with grants 92/C16646 and 316/C16648).

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

Associated awards:

C16646 A mechanistic study of the metabolisum of homocysteine by human endothelial cells; functional consequences for the vasculature

C16648 A mechanistic study of the metabolisum of homocysteine by human endothelial cells; functional consequences for the vasculature

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