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Use of vibrating multi-barrelled ion-selective electrodes to identify ion species permeating nonselective ion channels from plants
Reference
C16541
Principal Investigator / Supervisor
Professor Julia Davies
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Plant Sciences
Funding type
Research
Value (£)
192,436
Status
Completed
Type
Research Grant
Start date
15/04/2002
End date
14/02/2006
Duration
46 months
Abstract
The aim of this project is to enable for the first time identification of the physiologically relevant permeation of nonselective cation channels, thus enabling attribution of function (e.g. Ca2+ channel, Na+ channel) to several gene products. This will be done by using a vibrating multi-barrelled ion-selective electrode system to measure, in complex solutions, local depletion of Na+, K+ and Ca2+ ions near the plasma membrane of patch clamped Arabidopsis protoplasts transiently overexpressing a range of Arabidopsis ion channels (co-expressed with gfp to enable identification of successfully transformed protoplasts). Channel-GFP fusions will be used to identify membrane localisation of gene products using confocal microscopy.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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