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Lipid rafts and the spatial control of exocytosis

Reference	C16435
Principal Investigator / Supervisor	Professor Luke Chamberlain
Co-Investigators / Co-Supervisors	Professor Gwyn William Gould
Institution	University of Glasgow
Department	IBLS Division of Biochemistry & Molecula
Funding type	Research
Value (£)	231,328
Status	Completed
Type	Research Grant
Start date	01/07/2002
End date	01/07/2005
Duration	36 months

Abstract

We have shown that syntaxin 1A and SNAP-25 are highly enriched in lipid-raft fractions of PC12 cells. The raft- associated SNAREs exhibit distinct binding properties compared to non-raft associated SNAREs implying a distinct functional role of raft-associated SNAREs. Furthermore, we have shown that cholesterol depletion results in diminished secretion from PC12 cells. These observations suggest the hypothesis that raft-associated t-SNAREs play a crucial role in exocytosis. This proposal seems to capitalise on these novel observations. To that end we will identify how SNAREs are targeted to rafts to determine whether there are raft-associated SNAREs in primary neurones and use proteomic approaches to address specific aspects of neuronal raft function.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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