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Award details
Development of novel in vitro toxicity tests using human cell lines
Reference
C16419
Principal Investigator / Supervisor
Professor Michael Coleman
Co-Investigators /
Co-Supervisors
Institution
Aston University
Department
Sch of Life and Health Sciences
Funding type
Research
Value (£)
170,608
Status
Completed
Type
Research Grant
Start date
08/04/2002
End date
07/10/2005
Duration
42 months
Abstract
Quantitative functional capabilities of human cells in vitro will be used in vitro to provide indices of cell toxicity caused by stable drug metabolites. Human CaCo-2 cells, HL60 cells and isolated human neutrophils will be separated using a semi-permeable membrane from a source of stable toxic species generated by expressed human cytochrome P- 450 biotransformational enzymes as well as human hepatocytes bound to liverbeads. Toxicity will be determined in terms of changes in proline transport and GSSG/GSH ratio in the CaCo-2 cells, as well as changes in DPE-mediated caspase-3 activation in both HL60 cells and isolated human lymphocytes. These studies will both increase the human relevance of toxicity testing and reduce animal suffering.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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