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BspMI; a novel system for DNA recognition and cleavage
Reference
C16413
Principal Investigator / Supervisor
Professor Steve Halford
Co-Investigators /
Co-Supervisors
Institution
University of Bristol
Department
Biochemistry
Funding type
Research
Value (£)
201,108
Status
Completed
Type
Research Grant
Start date
01/02/2002
End date
31/05/2005
Duration
40 months
Abstract
BspMI differs from all other restriction enzymes identified to date. As a type IIs enzyme, it recognises an asymmetric DNA sequence and cleaves the DNA at fixed positions downstream of its site but, instead of being a monomer like other type IIs enzymes, BspMI is a tetramer that interacts with two sites before cleaving DNA. It can span two sites when these are separated by either relatively long lengths of DNA (viz. 700 bp) or very short lengths (viz. 12 bp). The latter are far too short for this type of DNA looping seen with several restriction enzymes. Instead, preliminary data on BspMI suggest that its interaction with two DNA sites involves extensive wrapping of the DNA around the tetrameric protein. The objective of this study is to identify the mode of action of BspMI, particularly with regard to the path of the DNA in its complexes with two DNA sites.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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