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Integration of antagonistic signals: role of protein-protein and protein-ligand interactions in nitrogen and carbon regulation
Reference
C15912
Principal Investigator / Supervisor
Professor Raymond Alan Dixon
Co-Investigators /
Co-Supervisors
Institution
John Innes Centre
Department
Molecular Microbiology
Funding type
Research
Value (£)
160,796
Status
Completed
Type
Research Grant
Start date
01/05/2002
End date
30/04/2005
Duration
36 months
Abstract
We have recently demonstrated that the signal transduction protein PII interacts with the NIFL-NIFA system to mediate nitrogen regulation. We propose to characterise this protein-protein interaction and study how the signal for nitrogen regulation is opposed in response to carbon status by the metabolite 2- oxoglutarate. We plan to use a combination of surface plasmon resonance (SPR), chemical cross-linking and mutagenesis to delineate the interaction surfaces in the PII-NIFL binary complex and determine how the formation and stability of this complex is influenced by uridylylation of the PII protein and ligand binding to both protein components.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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