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Molecular genetic studies of the Drosophila troponin-tropomyosin complex and its regulation of muscle contraction

Reference	C15904
Principal Investigator / Supervisor	Professor John Sparrow
Co-Investigators / Co-Supervisors	Professor Michael Geeves, Dr Justin Molloy, Dr Upendra Nongthomba
Institution	University of York
Department	Biology
Funding type	Research
Value (£)	209,628
Status	Completed
Type	Research Grant
Start date	15/10/2001
End date	15/11/2004
Duration	37 months

Abstract

Contraction of striated muscle is activated by Ca2+ binding to the troponin-tropomyosin (Tn-Tm) complex of the thin (actin) filament. Molecular details of how the Tn-Tm complex functions are still largely unknown. In a current BBSRC grant we have successfully recovered mutants in Tn, Tm and actin genes which affect muscle regulation of Drosophila flight muscles. To achieve our aim of using these to determine how the thin filament regulatory system works we propose expressing the proteins in E. coli for biochemical assays to investigate wild type and mutant proteins interactions and the activity of reconstituted thin filaments. We will also insert human Tnl cardiomyopathy mutants into Drosophila to look for further genetic interactions.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

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