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Project 'impossible': analysis of the cell cycle in synchronized Arabidopsis cells
Reference
C15792
Principal Investigator / Supervisor
Professor James Murray
Co-Investigators /
Co-Supervisors
Institution
University of Cambridge
Department
Institute of Biotechnology
Funding type
Research
Value (£)
237,096
Status
Completed
Type
Research Grant
Start date
01/12/2001
End date
01/12/2004
Duration
36 months
Abstract
Synchronized cultures are central to cell cycle studies, but have been difficult or impossible to obtain in Arabidopsis. We have developed the first synchronizable Arabidopsis cell suspension culture providing up to 80 percent S phase and 92 percent G2 phase synchrony. Our methods allow analysis both of the re-entry of cells into the cycle and progression of cells from S phase through mitosis. We propose the first genome-wide analysis of cell cycle gene regulation in a plant using a highly sensitive gene profiling technique. Analysis of genes for expression pattern and common regulatory pathways will allow them to be linked to other cellular processes. We will also analyse the activity of Arabidopsis cyclin- dependent kinases during the cell cycle.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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