Award details

Cooperativity of ligand binding at D2 dopamine receptors

Reference	C15778
Principal Investigator / Supervisor	Professor Philip Gordon Strange
Co-Investigators / Co-Supervisors
Institution	University of Reading
Department	Animal and Microbial Sciences
Funding type	Research
Value (£)	184,924
Status	Completed
Type	Research Grant
Start date	01/02/2002
End date	01/02/2005
Duration	36 months

Abstract

We have observed cooperativity in the binding of ligands to the D2 dopamine receptor which may be related to the recently described phenomenon of dimerisation of G protein coupled receptors. This project will extend these observations with particular emphasis on the generality and functional relevance of the observations. Cooperativity of ligand binding (equilibrium and kinetic assays) for a range of antagonists and agonists will be examined as well as the effects of several effectors of D2 receptor function such as sodium ions, guanine nucleotides and amiloride analogues. Functional relevance will be examined using the stimulation of [35S]GTP gamma S binding. Models of receptor function will be formulated and experiments designed to probe the models.

Summary

unavailable

Committee	Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics	X – not assigned to a current Research Topic
Research Priority	X – Research Priority information not available
Research Initiative	X - not in an Initiative
Funding Scheme	X – not Funded via a specific Funding Scheme

I accept the terms and conditions of use (opens in new window)

export PDF file

back to list new search