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Regulation of the cell surface dynamics of neuronal potassium channels: a role for ubiquitin- dependent mechanisms?
Reference
C15752
Principal Investigator / Supervisor
Dr Owen Jones
Co-Investigators /
Co-Supervisors
Institution
The University of Manchester
Department
Life Sciences
Funding type
Research
Value (£)
172,892
Status
Completed
Type
Research Grant
Start date
01/05/2002
End date
01/05/2005
Duration
36 months
Abstract
The contribution of discrete voltage-gated potassium channels (KChs) to nerve function reflects their densities over the cell surface. Even so, the mechanisms that regulate the surface densities of these, and other membrane proteins, are largely unknown. Based on the recent identification of ubiquitin conjugation as a pivotal cue for protein internalisation, and the co- incidence between internalisation and putative ubiquitination (UbE) motifs in Kvl.4 KChs, it is hypothesized that ubiquitination controls the cell surface residency of KChs. This project will test his notion by defining the ability, necessity and sufficiency of UbE motifs to direct the internalisation and ubiquitination of select KChs and reporter proteins in transfected cells. In parallel, interacting proteins, and thus control mechanisms, will be identified using the yeast two-hybrid system.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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