Award details

Comparative mechanistic studies on 1 zinc and 2 zinc metallo-beta-lactamases

ReferenceC15746
Principal Investigator / Supervisor Dr Timothy Walsh
Co-Investigators /
Co-Supervisors
Professor Anthony Clarke
Institution University of Bristol
DepartmentCellular and Molecular Medicine
Funding typeResearch
Value (£) 158,212
StatusCompleted
TypeResearch Grant
Start date 01/10/2001
End date 01/10/2004
Duration36 months

Abstract

This project will involve a detailed study of substrate binding and enzyme mechanism for three phylogenetically diverse metallo-beta-lactamases; L1, ImiS and IMP-1. This project is a synthesis of structural and kinetic information (steady- and pre-steady state) with a view to identifying how the reaction pathway is influenced by antibiotic (substrate) and enzyme structure. The data generated will extend and answer questions on existing hypotheses proposed by the project Group on their mechanistic pathways. The data generated will 1) be of value in future inhibitor design, 2) establish how metallo-beta-lactamases can hydrolyse a wide range of different substrates and 3) give an insight into other metallo-enzymes with similar active sites.

Summary

unavailable
Committee Closed Committee - Biochemistry & Cell Biology (BCB)
Research TopicsX – not assigned to a current Research Topic
Research PriorityX – Research Priority information not available
Research Initiative X - not in an Initiative
Funding SchemeX – not Funded via a specific Funding Scheme
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