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Award details
High pressure freezing to capture endoplasmic reticulum and Golgi dynamics
Reference
C15728
Principal Investigator / Supervisor
Professor Christopher Hawes
Co-Investigators /
Co-Supervisors
Institution
Oxford Brookes University
Department
Faculty of Health and Life Sciences
Funding type
Research
Value (£)
264,956
Status
Completed
Type
Research Grant
Start date
04/01/2002
End date
03/04/2005
Duration
39 months
Abstract
This application is to establish a high pressure freezing (HPF) facility at Brookes University through the purchase of a LEICA EMPACT freezer and to make the machine available to other BBSRC funded users. The key research programme to be supported by the technique is the dynamics of the plant secretory pathway concentrating on ER to Golgi transport currently being investigated in vivo with GFP technology. HPF will be used to capture dynamic events through freeze-fixation and freeze- substitution of plant material. This will be combined with selective impregnation and immuno-location techniques to study sub-domains within the ER for protein transport, Golgi biogenesis, regulation of Golgi stack number and the organisation of transport between the ER and Golgi.
Summary
unavailable
Committee
Closed Committee - Biochemistry & Cell Biology (BCB)
Research Topics
X – not assigned to a current Research Topic
Research Priority
X – Research Priority information not available
Research Initiative
X - not in an Initiative
Funding Scheme
X – not Funded via a specific Funding Scheme
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